Plasmid

Part:BBa_K4876002:Design

Designed by: Jin Luming   Group: iGEM23_Zhejiang-United   (2023-09-28)


colE1-cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 157
    Illegal NheI site found at 2638
    Illegal NheI site found at 11382
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4914
    Illegal BamHI site found at 5774
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5800
    Illegal NgoMIV site found at 10479
    Illegal NgoMIV site found at 11388
    Illegal AgeI site found at 243
    Illegal AgeI site found at 567
    Illegal AgeI site found at 6123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 11507
    Illegal SapI.rc site found at 8898
    Illegal SapI.rc site found at 9140


Design Notes

No


Source

No

References